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<br>Shiitake mushroom extract powder boasts polysaccharides, terpenoids, sterols, and lipids, some of which have immune-boosting, cholesterol-lowering, and anticancer effects. Shiitake are low in calories. According to statistics, thousands of women in the United States lose their lives each year due to cervical cancer. Recently, at the annual meeting of the Society of Gynecologic Oncology in Tampa, Dr. Smith of the University of Texas proposed that shiitake mushroom extracts contain active hexose-related compounds (AHCC) that could eradicate the human papillomavirus that causes cervical cancer in humans (HPV), and eradication of the virus (HPV) within 90 days. This compound (AHCC) also inhibits tumor growth. This discovery, like a beacon, illuminates a new way of treating cervical cancer. It is reported that cervical cancer is basically caused by HPV. Although the cervical cancer vaccine can be very effective in preventing viral infection, it can only prevent a part of the virus. The new treatment method of shiitake mushroom extract can reduce the incidence and mortality of cervical cancer.<br>
<br>In China and Japan, shiitake mushrooms have been highly valued for centuries for their nutritional and therapeutic properties. Now, scientists from the University of Granada (UGR) have shown that an extract of this fungus can reduce the virulence of one of the most antibiotic-resistant bacteria, Pseudomonas aeruginosa. This bacterium can be found in animals and plants and is thought to be responsible for many cases of acute infection. It usually occurs in human immunodeficiency virus (HIV)-infected patients, burn patients, critically ill patients, and especially those with underlying medical conditions. Such as chronic obstructive pulmonary disease (COPD), bronchiectasis and cystic fibrosis. It is not part of the human gut microbiome. However, exhaustion after antibiotic treatment promotes P. aeruginosa’. The UGR team has shown that this extract inhibits different virulence systems by forming biofilms. To this end, the team will continue to research alternative compounds that can use traditional antibiotics. 1. Used in health supplements. 2. Used in cosmetics & personal care products. 3. Used in beverages & snacks as a functional food additive. 1. It could boost heart health. The extract is rich in eritadenine, sterols and beta-glucans, wherein eritadenine inhibits an enzyme involved in producing cholesterol, sterols block cholesterol absorption in the gut, and beta-glucans lower cholesterol. The polysaccharide lentinan helps fight tumors by activating the immune system. In China and Japan, an injectable form of lentinan is used alongside chemotherapy and other major cancer treatments to improve immune function and quality of life in people with gastric cancer. Lentinan has also been shown to inhibit the growth and spread of leukemia cells.<br>
<br>Table 1: Scientific name, common name and sources of mushroom samples. Absolute acetone and absolute ethanol were offered by Guangzhou Chemical Reagent Factory (Guangzhou, China). 2-Diohenyl-1- picryhydrazyl (DPPH) was supplied by Shanghai Yuanye BiologicalTechnology Co., Ltd (Shanghai, China). Folin-Ciocalteu reagent was provided by Shanghai Sanjie Biotechnology Co., Ltd (Shanghai, China). Trolox was purchased from Aldrich Co. (St. Louis, MO, U.S.A.). Sodium carbonate was supplied by Tianjin Nuoke Technology Development Co., Ltd (Tianjin, China). Gallic acid was purchased from Tianjin Damao Chemical Reagent Co., Ltd (Tianjin, China). All the chemicals were of analytical grade. Each of the twenty dry mushroom samples was pulverized; 5.0 g of each mushroom powder was accurately weighed into a set of centrifuge tubes (each of the sample prepared in triplicate); 40 mL of absolute acetone was added into each tube for the preparation of acetone extracts. The samples were extracted for 24 hours and then centrifuged by a centrifuge (Weierkang Xiangying Centrifuge Co., Ltd, Changsha, Hunan, China) at 8000 rpm for 10 min.<br>
<br>The supernatants were transferred into new centrifuge tubes and stored at −20 degree C. The residues were dried by vaporizing and collected; 40 mL of absolute ethanol was added into the dry residues for the preparation of ethanol extracts. The same extraction procedures were repeated to prepare water extracts as acetone and ethanol extraction and finally hot water extraction was done at 99°C in water bath for 2 hours. All the extracts were stored at −20°C in the dark for further usage. For determination of organic extracts, 1 mL of each acetone extract, ethanol extract, blank and standard solutions was added into a pre-labeled test tube and mixed with 3 mL of DPPH solution. Fordetermination of aqueous extracts, 0.2 mL of each water extract, hot water extract, blank and standard solutions was added into a prelabeled centrifuge tube and mixed with 3.8 mL of DPPH solution. The mixtures were mixed evenly by vortexing and stood in the dark at room temperature for 20 min.<br>
<br>Then the mixtures were centrifuged by a centrifuge (Weierkang Xiangying Centrifuge Co., Ltd, Changsha, Hunan, China) at 3000 rpm for 10 min. The absorbance of each mixture was measured by an UV-Visible spectrophotometer (TI- 1901, Beijing Purkinje General Instrument Co., Ltd, Beijing, China) at 517 nm against solvent blank. If you have any sort of concerns pertaining to where and ways to utilize Supplier of shiitake mushroom extract powder for food Ingredients, you could call us at the web site. The results were expressed as Trolox equivalents (µmole of TE⁄g sample) in accordance to the standard curve of Trolox. For determination of water extracts, 50 µL of each water extract, hot water extract, blank and standard solution was mixed with 3 mL of distilled water, 250 µL of Folin-Ciocalteu reagent and 750 µL of 7% Na2CO3 solution and the mixtures were mixed evenly by vortexing. After standing at room temperature for 8 min, 950 µL of distilled water was added into each mixture and the mixtures were allowed to stand at room temperature for 1 hour. For organic solvent extraction, 500 µL of each acetone extract, ethanol extract, blank and standard solution was mixed with 2.55 mL of distilled water, 250 µL of Folin-Ciocalteu reagent and 750 µL of 7% Na2CO3 solution.<br> -
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